Journal: Cancer Medicine

Article Title: Methylseleninic acid elevates REDD1 and inhibits prostate cancer cell growth despite AKT activation and mTOR dysregulation in hypoxia

doi: 10.1002/cam4.198

Figure Lengend Snippet: Protein expression changes in cytosolic fractions of PC3, DU145, and PC-3M cells following MSeA treatments for 2 h, 6 h and overnight in hypoxia. The three prostate cancer cell types were stimulated with 10% FBS following an overnight starvation (0.1% FBS) in the presence and absence of MSeA in hypoxia (1% O 2 ). (A) Relatively increased levels of pAKT, REDD1 and pp70S6K (lower band) were observed after 2 h of MSeA treatment in invasive prostate cancer cells in hypoxia compared to respective untreated controls. (B) MSeA treatment after 6 h maintains elevated levels of pAKT in PC3 and PC-3M cells. REDD1 and pp70S6K levels induced by MSeA continue to increase in the three cell types at this time point. (C) An overnight exposure of invasive prostate cancer cells to MSeA maintained high levels of pAKT in PC3 and PC-3M cells while pAKT protein expression was marginally detectable in DU145 cells. The increase in REDD1 levels are more pronounced in the DU145 and PC-3M cells at this time point and pp70S6K expression was relatively higher in MSeA-treated cells as compared to untreated controls in each cell line. In addition, cleaved PARP appeared in a dose-dependent manner in each of the invasive prostate cancer cells following an overnight exposure with MSeA in hypoxia. (D) Following a 6 h treatment with MSeA in PC-3M cells, a concomitant dose-dependent elevation in pGSK3 β expression was observed. Fold change in band densities of phosphorylated proteins were normalized to the band densities of their respective native protein and to β -actin levels. While for pp70S6K, pGSK3 β and REDD1 the band densities were normalized to β -actin levels. FBS, fetal bovine serum; MSeA, methylseleninic acid; PARP, poly ADP-ribose polymerase; REDD1, regulated in development and DNA damage 1.

Article Snippet: However, a report by Schwarzer et al. in PC3 prostate cancer cells showed that knockdown of REDD1 gene was able to sensitize these cells toward apoptosis.

Techniques: Expressing

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Structure–activity relationship of C-17 ester derivatives of andrographolide in the A549 cell line.

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: Activity Assay

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Effect of compound 9s at various doses on the A549 cell line’s nuclear morphology when stained with DAPI. Cisplatin (17 μM) was used as a positive control.

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: Staining, Positive Control

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Effect of compound 9s with different concentrations over ROS generation in the A549 cell line using DCFDA dye. Cisplatin was taken as positive control (17 μM).

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: Positive Control

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Mitochondrial membrane potential was measured using Rh-123 dye on treatment with different concentrations of 9s in A549 cells. Cisplatin was used as positive control (17 μM).

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: Membrane, Positive Control

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Cytotoxic Activity (% Growth Inhibition) for Ester Derivatives of Andrographolide at 20 μM Concentration

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: Activity Assay, Inhibition, Concentration Assay

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Results of Screening Using the MTT Assay a

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: MTT Assay

Journal: ACS Omega

Article Title: Site-Selective Synthesis of C-17 Ester Derivatives of Natural Andrographolide for Evaluation as a Potential Anticancer Agent

doi: 10.1021/acsomega.3c00036

Figure Lengend Snippet: Expanded Panel of Lung Cancer Cell Lines with Respect to Selectivity Index with the Normal Cell Line

Article Snippet: A panel of human cancer cell lines procured from the European Collection of Authenticated Cell Cultures (ECACC) was used for this study, which included lung cancer A549, prostate cancer PC-3, colon cancer HCT-116, and breast cancer MCF-7.

Techniques: